The Neuroimaging and Electrophysiology Facility (NIEF) is a core research facility for confocal, multiphoton microscopy and electrophysiology applications. NIEF is also world-renowned as a Nikon Center of Excellence. We offer a range of services to meet your research needs, from the acquisition and analysis of fluorescence-based images and electrophysiology recordings to hands-on training in microscopy and electrophysiology. At the NIEF, you will have the opportunity to capture high-quality confocal images of subcellular structures and molecular events with great sensitivity and high speed as well as the detection of action potentials and ion channel currents from primary and recombinant cells to cellular tissues and animal models.

Of key importance is the facility’s capacity to detect fluorescence emissions from diverse biological preparations through confocal microscopy and two-photon microscopy while making electrophysiological measurements simultaneously. For instance, recent research provides examples of the power of coupling patch clamp electrophysiology techniques with calcium imaging. The facility provides training opportunities for students, postdoctoral fellows, and faculty in imaging techniques for the study of biological preparations. The NIEF is a completely self-sustained neuroimaging and electrophysiology research facility and a role model for Puerto Rico and the Caribbean.

We are dedicated to deliver a service of excellence and provide high-end technology in confocal microscopy and electrophysiology to support our common purpose of advancing science through research technical services.

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The NIEF was established and is supported by NIH grant 1P20 GM103642 01A1 and by University of Puerto Rico institutional funds.